%0 Journal Article
%T Establishment of Fibroblast Cell Line and Its Biological Characteristics in Matou Goat<br>马头山羊成纤维细胞系的建立与生物学特性分析
%A Tianda Li
%A Chousheng Liu
%A Zhigang Wang
%A Liping Zhang
%A Xiuzhu Sun
%A Junjin Zhao
%A Fei Meng
%A Guihe Luo
%A Jinqing Zhu
%A <br>李天达
%A 刘丑生
%A 王志刚
%A 张利平
%A 孙秀柱
%A 赵俊金
%A 孟飞
%A 罗桂河
%A 朱金清
%J 生物工程学报
%D 2008
%I 
%X Taking Matou goat ear margin as the study material, we succeeded in established a fibroblast cell line by the method of explant culture directly. Observations on morphology, dynamic growth, determination of viability, analysis of karyotype, test of microorganism and other characteristics were detected. Results showed: Population Doubling Time (PDT) of cells was approximately 36 h; Cell viability was 96.7% after thawing; The status of cell After passage was constant; Analysis of chromosomal karyotyps indicated that diploid (2n=60) account for 98% in the cell line. Every index in the cell line met all the standard quality controls of ATCC in USA. The established of Matou goat ear fibroblast cell line has not only important genetic resources preserved at the cell level, but also valuable material for genome, postgenome and somatic cell nuclear transfer research.
%K Matou goat
%K fibroblast cell line
%K cell culture
%K biological characteristics<br>马头山羊
%K 成纤维细胞系
%K 细胞培养
%K 生物学特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B527D13E314396C013AF88DF607C15D0&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=59906B3B2830C2C5&sid=112F428C63DFB16D&eid=5F72C4E06FEFC1BD&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=8