%0 Journal Article
%T A novel packaging system of recombinant AAV5/5 vector<br>新型重组AAV5/5载体及包装系统
%A Xiaoyan Dong
%A Wenhong Tian
%A Zhenhua Yuan
%A Shuping Tan
%A Xiaobing Wu
%A <br>董小岩
%A 田文洪
%A 袁振华
%A 谭淑萍
%A 吴小兵
%J 生物工程学报
%D 2010
%I 
%X We developed a scalable AAV5/5 vector packaging system by using replication competent recombinant herpes simplex type 1 virus as helper virus. The fragment containing rep and cap genes of AAV5 was inserted into the non-necessary gene (UL2) of HSV1 genome, resulting in the helper virus rHSV1-rep5cap5. An AAV5/5 vector pAAV5neo carrying two AAV5 ITRs was constructed by inserting a neo gene expression cassette into the plasmid backbone of pAV5CMV-GFP. pAAV5neo-EGFP was constructed by inserting EGFP gene into p...
%K Adeno-associated virus type 5 (AAV5)
%K herpes simplex virus I
%K virus vector
%K production system<br>5型腺相关病毒
%K (AAV5)，I型单纯疱疹病毒
%K (HSV1)，病毒载体，包装系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A5F57D5C22F84A2791BD6226661BA8C4&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=DC06EBDBAF4E06D3&eid=710C005323C0774A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0