%0 Journal Article
%T Generation of Mouse UBE2W Antibody and Analysis of UBE2W Expression in Mouse Tissues<br>小鼠泛素结合酶UBE2W的抗体制备及组织表达谱分析
%A Yingying Zhang
%A Hengqi Zhu
%A Lixia Zhao
%A Xiaowei Zhou
%A Peitang Huang
%A <br>张莹莹
%A 朱恒奇
%A 赵莉霞
%A 周晓巍
%A 黄培堂
%J 生物工程学报
%D 2008
%I 
%X Ubiquitin conjugating enzyme functions as the second enzyme required for protein ubiquitination and plays an important role in ubiquitin transferring and substrate specific recognition. UBE2W, a newly described member of E2 family, was formerly reported probably involving in phototransduction or retinal degeneration in Drosophila. In this study, we report that murine UBE2W harbors a typical UBC domain and is highly conserved in different vertebrate homologues. GST-tagged UBE2W was expressed in E. coli BL21 (DE3) and purified with GST affinity chromatography. Using this antigen, we generated and further separated rabbit polyclonal antibody of UBE2W, of which the activity and specificity were confirmed by immunoblotting of transiently expressed myc-UBE2W fusion protein. Wide expression of UBE2W was found in brain, muscle, heart, lung, liver, spleen, kidney and testis of mouse with the generated antibody, indicating the functional importance of this novel protein. Furthermore, the UBE2W highly expression was confined to the adult testis and was developmental stage-specific.
%K ubiquitin conjugating enzyme
%K UBE2W
%K antibody
%K tissue expression profile<br>泛素结合酶
%K UBE2W
%K 抗体
%K 组织表达谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B60BB7B2664EF252541BA4C09EFCFBA7&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=4002B7787911C73B&eid=385E3C2062167B88&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18