%0 Journal Article
%T Study on Vitrification of Porcine Embryos by Open Pulled Straw Method<br>猪胚胎开放式拉长细管玻璃化冷冻保存研究
%A ZHANG De-Fu
%A LIU Dong
%A WU Hua-Li
%A ZHENG Xiao-Feng
%A WANG Zhao-Kai
%A WANG Shao-Bing
%A <br>张德福
%A 刘东
%A 吴华丽
%A 郑筱峰
%A 王昭凯
%A 王少兵
%J 生物工程学报
%D 2006
%I 
%X 291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China.
%K pig
%K frozen embryos
%K vitrification<br>猪
%K 冷冻胚胎
%K 玻璃化冷冻
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E6C89B5BF071073F&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=450524CAAD043961&eid=8AD9BBE1FAF6BB78&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=23