%0 Journal Article
%T Cloning and Expression of Human Interleukin-26 in Escherichia coli<br>人白细胞介素26的基因克隆及其在大肠杆菌中的表达
%A LIU Yi-Qing
%A CHEN Zi-Jiang
%A ZHANG Xue
%A WANG Lai-Cheng
%A JIAO Yu-Lian
%A ZHANG Jie
%A MA Chun-Yan
%A CUI Bin
%A GAO Xin-Pu
%A LIU Zheng-Min
%A WU Kan
%A ZHAO Yue-Ran
%A <br>刘义庆
%A 陈子江
%A 张雪
%A 王来城
%A 焦玉莲
%A 张捷
%A 马春燕
%A 崔彬
%A 高新谱
%A 刘正敏
%A 吴侃
%A 赵跃然
%J 生物工程学报
%D 2006
%I 
%X To clone human interleukin-26 (hIL-26) and express it in E. coli efficiently. Two pairs of primers were synthesized according to the hIL-26 gene reported on GenBank. The hIL-26 gene was cloned by nest PCR following the first round RT-PCR from human peripherial blood monocytes total RNA, and then the PCR product was cloned into pMD18-T vector. Colony PCR, restriction analysis and sequence analysis showed that the gene cloned was the same as the reported hIL-26. The recombinant was cut with BamHI and EcoR I to obtain the hIL-26 fragment, and then the fragment was inserted into pBV220 which was cut with the same enzymes. The recombinant expression vector was induced to express hIL-26 at 42 degrees C, SDS-PAGE analysis showed that the recombinant protein accounted for up to 20% of the whole protein of E. coli, and the protein was also confirmed by Western blotting. Purity of the protein was found to be above 90% after purified with molecular sieve. After renaturalized with glutathione buffer, the promoting effect of it on the production of IFN-y in PBMC was detected by RT-PCR. A recombinant bacterial strain for expressing hIL-26 with biological activity was constructed successfully.
%K interleukin-26
%K cloning and expression
%K Escherichia coli
%K biological activity<br>白细胞介素-26
%K 克隆与表达
%K 大肠杆菌
%K 生物活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=80C4805BCCFF921B&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=38B194292C032A66&sid=09AA1448D1EAF9C1&eid=84A93BA251D28205&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=8