%0 Journal Article
%T The Effect of Oncolytic Adenovirus on Human Umbilical Vein Endothelial Cell<br>裂解型腺病毒Ad-E1A对人脐静脉内皮细胞的影响
%A YE Zhen-Min
%A WANG Xiao-Hua
%A ZHONG Jiang
%A MIAO Jing-Cheng
%A SHENG Wei-Hua
%A XIE Yu-Feng
%A WANG Jin-Zhi
%A YANG Ji-Cheng
%A <br>叶震敏
%A 汪小华
%A 钟江
%A 缪竞诚
%A 盛伟华
%A 谢宇锋
%A 王金志
%A 杨吉成
%J 生物工程学报
%D 2006
%I 
%X The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and cotransformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirns Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirns Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kB of ECV304 cells, indicating that AdE1A have potential of inhibition of tumor metastasis.
%K oncolytic adenovirus
%K Ad-E1A
%K human umbilical vein endothelial cell<br>裂解型腺病毒
%K Ad-E1A
%K 人脐静脉内皮细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B78D5C254BFAFD4D&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=38B194292C032A66&sid=21D8CE17EE5EE354&eid=EE23223CE4332BD7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=6