%0 Journal Article
%T Subcellular Localization and Tissues Expression Profile of hGPCRc: an Orphan G Protein-Coupled Receptor<br>孤儿G蛋白偶联受体hGPCRc的亚细胞定位及组织分布
%A YUAN Guang-Sheng
%A PAN Guang-Tang
%A WU Fang-Ming
%A HAN Chun-Guang
%A HUANG Huo-Gao
%A HU Ming
%A SHENG Li
%A CHEN Jing
%A LIU Yong-Xue Institute of Maize
%A Sichuan Agricultural University
%A Ya''''an
%A China Institute of Radiation Medicine
%A Academy of Military Medical Sciences
%A Beijing
%A China Navy General Hospital
%A Beijing
%A China
%A <br>袁广胜
%A 潘光堂
%A 吴芳明
%A 韩春光
%A 黄火高
%A 胡明
%A 盛莉
%A 陈静
%A 刘永学
%J 生物工程学报
%D 2005
%I 
%X As a member of orphan G protein-coupled receptors (oGPCRs), hGPCRc was cloned from human colon tissue and analyzed by bioinformatic softwares. It was showed that the corresponding amino acids of hGPCRc formed seven-transmembrane domains as the key characteristic of GPCRs. Then, the recombinant GFP-hGPCRc was constructed by fussing hGPCRc into pEGFP-N 1 carrying green fluorescent protein (GFP) gene, and CHO-K 1 cells were subsequently transfected with the GFP-hGPCRc or pEGFP-N 1. The green fluorescence protein expression in the two different transfected cells was observed under the laser scanning confocal microscopy (LSCM). It was showed that green fluorescence protein was distributed in the whole bodies of the cells transfected with pEGFP-N 1, but mainly distributed on the plasma membrane and cytoplasm membrane transfected with GFP-hGPCRc. Thus, the localization on the membrane of hGPCRc was accorded with the predication by bioinformatic analysis. The expression analysis of hGPCRc by RT-PCR indicated that hGPCRc was abundantly expressed in heart, kidney, cerebel and colon etc., but absent in liver, cerebra, small intestine and muscle etc.. The expressing profile of hGPCRc could provide some useful clues to understanding its effects on embryonic development and physiological functions.
%K orphan G protein-coupled receptors
%K hGPCRc
%K expression profile
%K CHO-K
%K 1cell line
%K subcellular localization<br>孤儿G蛋白偶联受体
%K hGPCRc
%K 组织分布
%K CHO-K1细胞系
%K 亚细胞定位
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C986066522D0AC57&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=38B194292C032A66&sid=683005D16807E4FE&eid=F7BB24011DC0D223&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=9