%0 Journal Article
%T Separation of Correctly Refolded and Mis-refolded Consensus Interferon by Hydrophobic Interaction Chromatography<br>疏水层析分离正确折叠与错误折叠的复合干扰素
%A Rong-Zhi Zhao
%A Yong-Dong Liu
%A Fang-Wei Wang
%A Jing-Jing Li
%A Xin Xia
%A Zhi-Guo Su
%A <br>赵荣志
%A 刘永东
%A 王芳薇
%A 李京京
%A 夏新
%A 苏志国
%J 生物工程学报
%D 2005
%I 
%X Hydrophobic interaction chromatography was used to separate correctly refolded and mis-refolded consensus interferon. The effects of ligand types, salt concentration, pH and flow rate were investigated. The best result could be obtained by using Butyl Sepharose 4 Fast Flow, 0.8 mol/L of ammonium sulfate, pH 8.3 and 90cm/h of linear flow rate. Reverse-phase HPLC analysis showed the purity of the pooled fraction was as high as 99.6%. The specific activity of purified consensus interferon was 2.3 x 10(9) IU/mg, The mass recovery of targeth protein was 36.7%.
%K consensus interferon
%K purification
%K hydrophobic interaction chromatography<br>复合干扰素
%K 纯化
%K 疏水层析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EB0EF27B9D49B654&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=38B194292C032A66&sid=9B95A71E6639C039&eid=366B1248A15658C5&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=9