%0 Journal Article
%T Dissociated mouse tooth germ epithelial cells retain the expression of tooth developmental genes during reaggregation process<br>解离的小鼠牙胚上皮细胞在重聚发育过程仍维持牙齿发育基因的表达
%A Xuefeng Hu
%A Chensheng Lin
%A Bingmei Wang
%A Pingping Han
%A Yanding Zhang
%A <br>胡雪峰
%A 林陈胜
%A 王冰梅
%A 韩萍萍
%A 张彦定
%J 生物工程学报
%D 2010
%I 
%X Generation of bio-engineered teeth by using stem cells will be a major approach for bioengineered implantation. Previous studies have demonstrated that dissociated tooth germ cells are capable of generating a tooth after reaggregation in vitro. However, the cellular and molecular mechanisms underlying this tooth regeneration are not clear. In this study, we dispersed E13.5 molar germ into single cells, immediately reaggregated them into cell pellet, then grafted the reaggregates under mouse kidney capsule for various times of culture. We investigated the morphogenesis and the expression of several developmental genes in dental epithelial cells in reaggregates of tooth germ cells. We found that dissociated tooth germ cells, after reaggregation, recapitulated normal tooth developmental process. In additon, dissociated dental epithelial cells retained the expression of Fgf8, Noggin, and Shh during reaggregation and tooth regeneration processes. Our results demonstrated that, despite of under dissociated status, dental epithelial cells maintained their odontogenic fate after re-aggregation with dental mesenchymal cells. These results provided important information for future in vitro generation of bio-engineered teeth from stem cells.
%K tooth regeneration
%K reaggregation
%K dental epithelium
%K gene expression<br>牙齿再生，细胞重聚，牙上皮，基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EC2D3E571EF486DD30D32F08011B30F2&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=59906B3B2830C2C5&sid=721F8E311AAA0176&eid=4B35F43975EC564A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0