%0 Journal Article
%T Optimization of enzymatic preparation of glucose 1-phosphate by response surface methodology<br>酶法制备1-磷酸葡萄糖合成条件的响应曲面法优化
%A 王晓娟
%A 靳利娥
%A 畅芬芬
%A 阎果兰
%J 生物工程学报
%D 2013
%I 
%X With glucose as substrate, sodium tripolyphosphate as the phosphorus acylating agent, and phosphorylase of Solanum tuberosum as the catalyst, glucose 1-phosphate was synthesized. Based on a three-level, three-variable Box-Behnken experimental design, response surface methodology was used to evaluate the effects of temperature, molar ratio of glucose to sodium tripolyphosphate and time on the production. The structure of the product was confirmed by 1H NMR spectra. The results show that the optimum conditions were as follows: temperature 35 °C, molar ratio of glucose to sodium tripolyphosphate 1.35:1 and time 19 h.
%K phosphorylase of Solanum tuberosum
%K glucose 1-phosphate
%K response surface methodology<br>马铃薯磷酸化酶，1-磷酸葡萄糖，响应曲面法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2DB3B6D3544EDEBDC9817178EA3624BC&yid=FF7AA908D58E97FA&vid=771469D9D58C34FF&iid=CA4FD0336C81A37A&sid=D767283A3B658885&eid=4DB1E72614E68564&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0