%0 Journal Article
%T Construction and Characterization of a Recombinant Fowlpox Virus Co-expressing F, HN Genes of Newcastle Disease Virus and gB Gene of Infectious Larygnotracheitis Virus<br>表达鸡新城疫病毒F、HN基因和传染性喉气管炎病毒gB基因的重组鸡痘病毒的构建及其特性研究
%A SUN Hui-Ling
%A WANG Yun-Feng
%A MIAO De-Yuan
%A ZHANG Pei-Jun
%A ZHI Hai-Dong
%A XU Ling-Long
%A WANG Mei
%A TONG Guang-Zhi
%A WANG Ming
%A <br>孙惠玲
%A 王云峰
%A 苗得园
%A 张培君
%A 智海东
%A 徐灵龙
%A 王玫
%A 童光志
%A 汪明
%J 生物工程学报
%D 2006
%I 
%X The Fusion (F) and Haemagglutinin-Neuraminidase (HN) genes of Newcastle disease virus (NDV) and the glycoprotein B (gB) gene of infectious laryngothracheitis virus (ILTV) as well as a LacZ reporter gene were all inserted into a nonessential gene of fowlpox virus (FPV) 017 strain by homologous recombination. The NDV and ILTV genes were each under the control of a fowlpox virus immediate early/late promoter (LP2EP2) while the LacZ reporter gene expression cassette was regulated by a P11 late promoter. A recombinant FPV harboring the F,HN and gB genes as well as the LacZ gene,designated as rFPV-F/HN/gB/LacZ,was obtained after ten cycles of blue plaque purification. The presence of the NDV and ILTV genes was confirmed by PCR. The expression of the recombinant proteins in rFPV-F/HN/gB/LacZ were characterized by Western blot (F and gB proteins) and indirect immunofluorescence test (F,HN and gB proteins). The results demonstrated that all four foreign proteins,which were encoded within a 10kb gene fragment,could be expressed authentically and efficiently. Compared to the parental virus,rFPV-F/HN/gB/LacZ showed no obvious difference with respect to virus replication and cytopathogenic effects in chicken embryo fibroblasts (CEF) cell culture. Overall,our work suggests that FPV can be a useful live virus vector for the expression of multi- foreign genes against multiple avian pathogens.
%K recombinant fowlpox virus
%K Newcastle disease virus
%K infectious laryngothracheitis virus
%K F gene
%K HN gene
%K gB gene<br>重组鸡痘病毒
%K 新城疫病毒
%K 传染性喉气管炎病毒
%K F基因
%K HN基因
%K gB基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FD12291399958E87&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=B31275AF3241DB2D&sid=90EAFEE49150CFCD&eid=384C451D3BE0C38C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=24