%0 Journal Article
%T Application of Optical Protein-chip in Detecting Phage M13KO7<br>光学蛋白芯片技术在噬菌体M13KO7检测中的应用
%A Cai QI
%A Jing FENG
%A Zhan-Hui WANG
%A Yong-Hong MENG
%A Xi-Yun YAN
%A Gang JIN
%A <br>齐财
%A 冯静
%A 王战会
%A 孟永宏
%A 阎锡蕴
%A 靳刚
%J 生物工程学报
%D 2006
%I 
%X Avidin layer was bound on the substrate surface of Silicon wafer modified with aldehyde. The interaction between avidin and biotin was adopted for the immobilization of mouse monoclonal biotin-anti-M13 (antibody GP3)-labeled biotin. The surface was incubated in a solution containing phage M13KO7, which was trapped by the antibody GP3 with the interaction between phage M13KO7 and antibody GP3, resulting in a variation of layer thickness that was detected by imaging ellipsometry. The results showed a saturated layer of antibody GP3 with a thickness about 6.9 nm on the surface of the silicon wafer. The specific interaction between phage M13KO7 and antibody GP3 resulted in a variation of the layer thickness. The layer of phage M13KO7 bound with antibody GP3 was 17.5 nm in the concentration of 1.1 x 10(10) pfu/mL. Each variation of layer thickness corresponded to a concentration of phage M13KO7 in the range of 0.1 x 10(10) approximately 2.5 x 10(10) pfu/mL, with the sensitivity of 10(9) pfu/mL. Compared with other methods, the optical protein-chip requires only short measurement time, is label free, is a quantitative test, and can be visualized. This study could be significant on the investigation of interactions between the antibody and virus, and shows potential in the early diagnosis of virosis.
%K optical protein chip
%K imaging ellipsometry
%K phage M13KO7
%K antibody GP3<br>蛋白质芯片
%K 生物传感器
%K 噬菌体
%K GP3抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=12FA3ED03977A6EB&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=353B961D86F026C0&eid=E9F71A2A3584AD5D&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17