%0 Journal Article
%T Expression of the Infectious Bursal Disease Virus Polyprotein in Vero Cells Using Attenuated Salmonella typhimurium as Transgenic Carrier<br>减毒沙门氏菌为载体在Vero细胞中表达传染性法氏囊病病毒多聚蛋白基因
%A LI Long
%A FANG Wei-Huan FAN Yong-Jun XU Jian FANG Li LI Jian-Rong YU Lian
%A <br>李龙
%A 方维焕
%A 樊拥军
%A 许健
%A 方立
%A 李建荣
%A 于涟
%J 生物工程学报
%D 2004
%I 
%X To examine if polyprotein gene(VP2/VP4/VP3) of Infectious Bursal Disease Virus (IBDV) could be delivered into mammalian cells and expressed using attenuated Salmonella typhimurium as vector. The IBDV polyprotein gene was amplified by RT-PCR and inserted in to pCI, an eukaryotic expression plasmid. The resulting recombinant pCI-VP2/VP4/VP3 was transformed by electroporation into attenuated Salmonella typhimurium strain ZJ111 (dam - and phoP -), which was then use to transfect the Vero cells. Gene specific RT-PCR revealed that VP2/VP4/VP3 was transcribed into mRNA in the Vero cells. Indirect immunofluorscence assay, SDS-PAGE and Western-blot analysis showed that VP2/VP4/VP3 was expressed and the product was immuno-reactive with anti-IBDV serum. This work provides essential precondition for developing a new oral DNA vaccine against IBDV.
%K infectious bursal disease virus
%K polyprotein
%K attenuated Salmonella typhimurium
%K DNA vaccine delivery vector<br>鸡传染性法氏囊病病毒，
%K 多聚蛋白，
%K 减毒沙门氏菌，
%K 基因疫苗载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=871293DDB97DBEFB&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=38B194292C032A66&sid=B40AD8FE6FA88DE9&eid=78976D931AD1540F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=14