%0 Journal Article
%T Protein Array Technology Applied in High Throughput Monoclonal Antibody Generation<br>蛋白质芯片技术应用于高通量单克隆抗体制备研究
%A SONG Kai
%A YE Sai
%A ZHOU Jia-Jing
%A PENG Hai-Lin
%A WANG Sheng-Nian
%A WEI Ling
%A XIAO Hua-Sheng
%A ZHAO Guo-Ping
%A ZHANG Qing-Hua
%A <br>宋凯
%A 叶赛
%A 周佳菁
%A 彭海林
%A 王升年
%A 卫玲
%A 肖华胜
%A 赵国屏
%A 张庆华
%J 生物工程学报
%D 2007
%I 
%X To reduce the huge labor-cost in the screening in traditional monoclonal antibody generation, We established a new system for monoclonal antibody generation integrating with protein array. BALB/c mice were immunized by eight recombinant proteins respectively, and the positive hybridoma cells were obtained by cell fusion and ELISA screening. All the eight kinds of positive hybridoma cells were mixed, cloned, screened by protein array, and definite dilution cloned. Results: 175 single cell clones were obtained by complex cloning, and 119 of those were positive clones. Then 8 positive cell lines were generated by the following 2 rounds definite dilution cloning. By comparing with the traditional method, we got 8 monoclonal antibodies using the combined protein array screening and multiplex cloning method in 1 cycle, and fewer amounts of antigens were used. As a result, the combined protein array and multiplex cloning method could be used as an economical, rapid and simple tool applying in high throughput monoclonal antibody generation.
%K protein array
%K monoclonal antibody
%K cloning
%K screen<br>蛋白质芯片
%K 单克隆抗体
%K 克隆化
%K 筛选
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=54AB0179FE3A3A7D13D1A071658AAB40&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=76C32027E03E49D7&eid=9C76A7541E963918&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=8