%0 Journal Article
%T Immune response and immunoprotection of pompanos (Trachinotus ovatus) against Cryptocaryon irritans<br>卵形鲳鲹对刺激隐核虫的免疫应答和免疫保护研究
%A 但学明
%A 李安兴
%A 林小涛
%A 柏建山
%J 水生生物学报
%D 2008
%I 
%X The humoral immune responses and host protection of pompanos (Trachinotus ovatus), against Cryptocaryon irritans were determined after immunization with live theronts and killed theronts of. C. irritans. Two groups of pompanos (150 animals per group) were immunized three times with live theronts of C. irritans by immersion at a dose of 8×103 theronts per fish or with killed theronts of C. irritans by intraperitoneal (i.p.) injection at a dose of 1.5×105 theronts per fish. The immobilization titers of antiserum and skin culture supernatant of the immunized fish were determined with an immobilization assay every two weeks. Also the immunized fish were challenged with a sublethal dose of theronts (6×103 theronts per fish) or lethal dose of theronts (2×104 theronts per fish) in Week 14.The level of C. irritans infection (indicated by relative infection level, RII) and survival of pompanos were determined after C. irritans challenge. The results showed that the specific immobilizing antibodies could be detected in both antiserum and skin culture supernatant in the immunized fish. Both immunization methods (immersion with live theronts or i.p. injection with killed theronts) could confer protection to fish because when all fish were challenged with a lethal dose of theronts of C. irritans, some of the immunised fish survived, but all control fish died. There was a positive correlation between higher levels of the specific immobilizing antibodies and host survival rate in the immunized fish. However, compared with i.p. injection with killed theronts, immersion with live theronts was more effective in stimulating immune responses, which indicated by the higher immobilization titers in both antiserum and skin culture supernatant and the higher survival rate of the immunised fish. When fish immunised with live theronts and killed theronts were challenged with a sublethal dose of theronts of C. irritans, the reduction rate of RII of C. irritans were 50.6% and 37.2% respectively. When fish were challenged with a lethal dose of theronts of C. irritans, the survival rate of fish immunised with live theronts and killed theronts were 60% and 40% respectively. In both groups of fish, antibody levels in skin culture supernatant were much lower than those in serum. The immobilizing antibodies in serum and the immobilizing antibodies in skin culture supernatant of the immunized fish were different in terms of onset of immunity, timing and pattern of the peaks, and immobilization titers. Therefore, it is speculated that pathway of the systemic immune responses and mucosal immune responses were different. The present study indicates that humoral immune response, especially mucosal immune response, plays an important role in protection against C. irritans infection. Vaccination may be an effective way to prevent white spot disease of marine fish.
%K 免疫应答
%K 免疫保护
%K 主动免疫
%K 刺激隐核虫
%K 卵形鲳鲹
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=18CBE13D1370AEDB26F838345948B5EF&yid=67289AFF6305E306&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=FC0714F8D2EB605D&eid=13553B2D12F347E8&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=1&reference_num=0