%0 Journal Article
%T Metabolism of Recombinant CHO-GS Cell Reducing of Toxic Effect of Ammonia<br>重组CHO-GS细胞降低氨毒副作用的代谢研究
%A ZHANG Fang
%A YI Xiao-Ping
%A SUN Xiang-Ming
%A ZHANG Yuan-Xing
%A <br>张芳
%A 易小萍
%A 孙祥明
%A 张元兴
%J 生物工程学报
%D 2006
%I 
%X The toxic effect of ammonia on rCHO-GS cell decreased obviously due to the transfection of GS system in serum-free culture. The maximum cell density, 15.6 x 10(5) cells/mL was obtained in the culture with 1.42 mmol/L ammonia. The growth of rCHO-GS cell was inhibited with an increased ammonia concentration. However, a cell density of 8.9 x 10(5) cells/mL was obtained when the concentration of ammonia was 12.65mmol/L. The intracellar metabolic pathways were affected due to the decrease of the toxic effect of ammonia on rCHO-GS cell. With the increase of initial ammonia concentration from 0.36mmol/L to 12.65mmol/L, the yield coefficients of cell to glucose and lactate to glucose decreased. The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) increased by 43%, 140% and 25%, respectively, indicating that the utilization of glucose increased and the glycolysis pathway was more prone to efficient energy metabolism pathway. An increased activity of glutamate-pyruvate aminotransferase (GPT) showed that the conversation from glutamate to alpha-ketoglutarate was shifted to glutamate-pyruvate transamination pathway. The deamination pathway was inhibited due to a decreased activity of glutamate dehydrogenase. In addition, the number of cells in G0/G1 phase increased and the specific production rate of recombinant protein increased by 2.1-fold with the increase of initial ammonia concentration from 0.36mmol/L to 12.65mmol/L.
%K rCHO cell
%K glutamine synthetase
%K serum-free medium
%K metabolism
%K ammonia<br>重组CHO细胞
%K 谷氨酰胺合成酶
%K 无血清培养
%K 代谢
%K 氨
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0A225B918B2AC9B2&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=BB0EA31DB1B01173&eid=8C83C265AD318E34&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=26