%0 Journal Article
%T Recombinant Aspergillus niger Glucose Oxidase Expressed in Trichoderma reesei<br>瑞氏木霉表达黑曲霉葡萄糖氧化酶
%A MU Jing-Yu
%A WANG Qiao
%A YANG Daniil
%A WANG En-Si
%A WANG Qing
%A HUANG Yue
%A <br>母敬郁
%A 王峤
%A 杨纯中
%A 王恩思
%A 王清
%A 黄跃
%J 生物工程学报
%D 2006
%I 
%X It was expected that recombinant Aspergillus niger glucose oxidase could be expressed in Trichoderma reesei with stable activity. T. reesei CBHI promoter--CBHI ss. gene--A. niger glucose oxidase gene--T. reesei CBHI terminator--A. nidulans gpd promoter--E. coli Hygromycin B phosphotransferase gene--A. nidulans trpC terminator--pUC19 (pCBHGOD) vector was constructed in E. coli DH5alpha by PCR application and gene cloning methods. T. reesei QM9414 protoplast was transformed by T. reesei CBHI promoter-CBHI ss. Gene--A. niger glucose oxidase gene--T. reesei CBHI terminator-A. nidulans gpd promoter--E. coli Hygromycin B phosphotransferase gene--A. nidulans trpC terminator linear DNA fragment (CBHGOD fragment) that was made by digestion of pCBHGOD with Kpn I. T. reesei mutant clone with homologous recombinant A. niger glucose oxidase gene was selected by PCR method. Recombinant glucose oxidase was produced by mutant T. reesei strain under induction of wheat straw for 5 days. Recombinant glucose oxidase molecular mass was showed the same as native A. niger glucose oxidase standard from Sigma company by Western blot analysis. Recombinant glucose oxidase activity was 25u/mL in medium. The yield was 0.5 g/L in comparison with Sigma company glucose oxidase standard. There was no recombinant GOD degradation during Trichoderma reesei cultivation that was showed in Western blot analysis. Trichoderma reesei has capability to be a new recombinant host for Aspergillus niger GOD production.
%K Trichoderma reesei
%K Aspergillus niger
%K glucose oxidase
%K homologous recombination<br>瑞氏木霉
%K 黑曲霉
%K 葡萄糖氧化酶
%K 同源重组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4A26166244981C94&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=0D0D661F0B316AD5&eid=7AA74D31F1FF2DCE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=21