%0 Journal Article
%T Secreted Expression of the Combinant Antimicrobial Peptide PL in Pichia pastoris and Its Antibacterial Activity in vitro<br>复合抗菌肽PL在毕赤酵母中的分泌表达及其活性研究
%A NIU Ming-Fu
%A LI Xiang
%A CAO Rui-Bing
%A ZHOU Bin
%A CHEN De-Sheng
%A CHEN Pu-Yan
%A <br>牛明福
%A 李翔
%A 曹瑞兵
%A 周斌
%A 陈德胜
%A 陈溥言
%J 生物工程学报
%D 2007
%I 
%X In order to obtain a high activity antibacterial peptide, An expression vector pPICZalphaA-pl is constructed with a tandem of four antimicrobial peptides in the same direction,which includes Protegrin-1 (PG-1), Scorpion Defensin (SD), Metalnikowin-2A and Sheep Myeloid Antibacterial Peptide (SMAP-29) (serial number in GenBank are AAB27599, AAAB27538, P80409 and P49928 respectively). At the same time the expression vector pPICZalphaA-sd which express Scorpion Defensin was contructed. The expression vectors of pPICZalphaA-pl and pPICZalphaA-sd were linearized and transformed into the yeast host strain X-33 respectively. Under the control of the promoter AOX1 (alcohol oxidase1), the peptides PL and SD were secreted expressed. Their heat-stable property, acid-stable property and MIC were detected in vitro. The results suggest the peptides PL and SD have good heat-stable and acid-stable properties, and the combinant PL peptide showes higher antibacterial activity against several Gram-positive bacteria (G+) and Gram-negative bacteria (G-) than the peptide SD, especially against Escherichia coli. The antibacterial activity of combinant antimicrobial peptide PL shows its far exploiting perspective.
%K recombinant antimicrobial peptide PL
%K scorpion defensin(SD)
%K secretion expression
%K heat and stable stability
%K antibacterial activity<br>复合抗菌肽PL
%K 蝎子防御素SD
%K 分泌表达
%K 热酸稳定性
%K 抑菌活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=497017A8E39F16B2&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=6B3068A7C27BD349&eid=B941678158018439&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=13