%0 Journal Article
%T Cloning and Expression of a New Glucoamylase Gene<br>一个新葡萄糖淀粉酶基因的克隆与表达
%A YANG Li-Quan
%A DAI Xiao-Jun
%A LUO Yuan-Ming
%A MA Chun-Xiao
%A HOU Jian-Hu
%A WU Zhi-Qiang
%A WANG Cui-Yan
%A LI Ming-Gang
%A <br>杨立泉
%A 戴小军
%A 罗元明
%A 马春晓
%A 侯建华
%A 武志强
%A 王翠艳
%A 李明刚
%J 生物工程学报
%D 2007
%I 
%X According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
%K Rhizopus arrhizu
%K glucoamylase
%K clone
%K expression
%K Picha pastoris<br>少根根霉
%K 葡萄糖淀粉酶
%K 克隆
%K 表迭
%K 毕赤酵母
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FAB7005BA355FCCD&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=F18BA6286A889C1C&eid=BE5DBC360CD4FFB9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=16