%0 Journal Article
%T XBP-1 Interacts with Estrogen Receptor α(ERα)<br>XBP-1和Erα存在相互作用
%A Li-Hua Ding
%A Qi-Nong Ye
%A Jing-Hua Yan
%A Jian-Hua Zhu
%A Qiu-Jun Lü
%A Zong-Hua Wang
%A Cui-Fen Huang
%A <br>丁丽华
%A 叶棋浓
%A 严景华
%A 朱建华
%A 吕秋军
%A 王宗华
%A 黄翠芬
%J 生物工程学报
%D 2004
%I 
%X Estrogen receptor alpha (ERalpha) has been a primary target of treatment as well as a prognostic indicator for breast cancer. The level of human X-box binding protein 1 (XBP-1) mRNA was related with that of ERalpha in breast tumors and was over-expressed in some breast tumors. These previous studies suggested that XBP-1 may interact with ERalpha. XBP-1 has two isoforms, XBP-1S and XBP-1U, as the result of unique splicing. GST pull-down assay showed that both XBP-1S and XBP-1U bound to ERalpha in vitro. The binding of XBP-1S to ERalpha was stronger than that of XBP-1U to ERalpha. Co-immunoprecipitation revealed that the binding was in a ligand-independent manner. XBP-1S and XBP-1U interacted with the region of ERalpha that contains a DNA-binding domain. The ERalpha-interacting regions on XBP-1S and XBP-1U have been mapped to two regions, the N-terminal basic region leucine zipper domain (bzip) and the C-terminal activation domain. These findings suggest that XBP-1S and XBP-1U may participate in ERalpha signaling pathway through the mediation of ERalpha.
%K estrogen receptor
%K X-box binding protein 1(XBP-1)
%K interaction<br>雌激素受体α(Erα)，
%K X盒结合蛋白1（XBP-1），
%K 相互作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6D5790607E652050&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=38B194292C032A66&sid=2E01F39B6CBD53DE&eid=50EA2A80A7D254EF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=26