%0 Journal Article
%T Medium Optimization by Response Surface Method for Transgenic Synechococcus sp. PCC 7002 with Mouse Metallothionein-I Gene<br>利用响应面方法优化转小鼠金属硫蛋白-I基因聚球藻7002的培养基成分
%A Wenlu Zeng
%A Feifei Zhao
%A Zhaogen Cao
%A Binggen Ru
%A <br>曾文炉
%A 赵飞飞
%A 曹照根
%A 茹炳根
%J 生物工程学报
%D 2008
%I 
%X To implement high cell density cultivation of transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-I gene, and its applications in heavy metal wastewater, we optimized the medium components. We used response surface method, a useful tool for multi-factor process optimization, through a three- step experiment design: full factor design, the steepest climbing and central composite design. The selected three factors were NaHCO3, NaNO3, and initial pH. The optimized medium contained 1.696 g/L NaHCO3 and 8.57 g/L NaNO3, at initial pH 8.57. The optimized medium was validated in 2-L and 20-L air lift photo-bioreactor, respectively. The maximum cell concentration reached 4.16 g/L for 2-L bioreactor, and 3.12 g/L for 20-L bioreactor.
%K mouse metallothionein-I
%K Synechococcus sp
%K PCC 7002
%K transgenic
%K response surface method
%K optimization<br>小鼠金属硫蛋白-I
%K 聚球藻7002
%K 转基因
%K 响应面方法
%K 优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=1041DE7E0EB914A5F7807630E3B56BF5&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=B47A0E731AF43EB2&eid=58F693790F887B3B&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17