%0 Journal Article
%T Development of a fed-batch process for TNFR-Fc producing GS-CHO cells<br>表达TNFR-Fc融合蛋白的GS-CHO细胞动态流加培养过程的设计
%A Li Fan
%A Liang Zhao
%A Yating Sun
%A Tianci Kou
%A Wensong Tan
%A <br>范里
%A 赵亮
%A 孙亚婷
%A 寇天赐
%A 谭文松
%J 生物工程学报
%D 2010
%I 
%X TNFR-Fc is an important fusion protein that has great potential in therapeutic and diagnostic applications. We developed an efficient fed-batch process for GS-CHO cells to produce TNFR-Fc. The rationale of this fed-batch process relies on the supply of sufficient nutrients to meet the requirements of cell metabolism. The optimal feed medium was designed through ration design. A metabolically responsive feeding strategy was designed and dynamically adjusted based on the residual glucose concentration determined off-line. In this process, the maximal viable cell density and antibody concentration reached above 9.4×106 cells/mL and 207 mg/L, respectively. Compared with the batch process, the newly developed fed-batch process increased the cell yield by 3.4 fold and the final antibody concentration by 3 fold. This fed-batch process would therefore facilitate the production of therapeutic antibody by GS-CHO cells.
%K GS-CHO cell
%K fed-batch
%K TNFR-Fc
%K glucose
%K dynamic model<br>GS-CHO细胞，流加培养，TNFR-Fc融合蛋白，葡萄糖，动力学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CF8DFC94BAEA3FAFC48ADEE11F185968&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=CEC789B3C68C3BB3&eid=8B59EA573021D671&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0