%0 Journal Article
%T Expression of GP5-M Fusion Protein of Porcine Reproductive and Respiratory Syndrone Virus(PRRSV) and Establishment of ELISA Diagnose Based on the Recombinant Fusion Protein<br>猪繁殖与呼吸综合征病毒(PRRSV)GP5-M在大肠杆菌中的融合表达及其ELISA诊断方法的建立
%A JIANG Yun-Bo
%A FANG Liu-Rong
%A XIAO Shao-Bo
%A XIE Tian-Tian
%A CHEN Huan-Chun Laboratory of Animal Virology
%A College of Animal Science
%A Veterinary Medici ne
%A Huazhong Agriculture University
%A Wuhan
%A China
%A <br>江云波
%A 方六荣
%A 肖少波
%A 谢甜甜
%A 陈焕春
%J 生物工程学报
%D 2005
%I 
%X The cDNA fragment encoding the truncated GP5 and the full-length M pr o tein of Porcine Reproductive and Respiratory Syndrone Virus(PRRSV)were orderly fused to the downstream of glutathione S-transferase(GST) of pGEX-KG expressi on vector, resulting in the fusion expression plasmid pKG-56. After transformed in to E.coli BL21(DE3)and induced by IPTG, the results of SDS-PAGE showed t hat t he GST-GP5-M fusion protein was expressed in high level. Western-blot was per for med to confirm that the expressed fusion protein could specifically react with a ntiserum against PRRSV. The fusion protein was further purified and used as an a ntigen to establish a novel PRRSV ELISA diagnose assay(P56-ELISA). Comparison between P56-ELISA and the abroad kit IDEXX-ELISA showed the two methods had 94 .1 percent agreement by detecting 205 serum samples, indicating that the indirect P56-ELISA was specific and sensitive. The correlation between virus neutralization antibody of the infected pigs (not convalescent pigs) and a ntibody response to the fusion protein GP5-M was further studied.The regressio n function analysis suggested that there was no significant correlation between E LISA antibody response (OD 630nm) to the fusion protein GP5-M in cli nical serum and their specific neutralizing titers.
%K porcine reproductive and respiratory syndrome virus
%K the fusion protein GST-GP5-M
%K ELISA
%K neutralization antibody
%K correlation<br>繁殖与呼吸综合征病毒
%K GP5-M融合蛋白
%K ELISA
%K 中和抗体
%K 相关性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C2A7734A33D093BE&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=0B39A22176CE99FB&sid=0F7768518993EDDE&eid=7ABC4505E3960D2B&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=6&reference_num=19