%0 Journal Article
%T Cloning and Sequence Analysis of cDNA Encoding Porcine αv Subunit for FMDV Receptor<br>猪口蹄疫病毒受体通用亚基αv的基因克隆及序列分析
%A DU Jun-Zheng
%A GAO Shan-Dian
%A CHANG Hui-Yun
%A CONG Guo-Zheng
%A SHAO Jun-Jun
%A LIN Tong
%A CAI Xue-Peng
%A XIE Qing-Ge
%A <br>独军政
%A 高闪电
%A 常惠芸
%A 丛国正
%A 邵军军
%A 林彤
%A 才学鹏
%A 谢庆阁
%J 生物工程学报
%D 2007
%I 
%X Receptors play a crucial role in determining the pathogenesis and tissue tropism of virus. Foot-and-mouth disease virus (FMDV) has been showed to use four integrins, alphavbeta1, alphavbeta3, alphavbeta6 and alphavbeta8 as receptors to initiate infection. In this study, the porcine integrin alphav gene was cloned by RT-PCR from the lung tissue of healed pig infected experimently with FMDV, and compared its nucleotide and deduced amino acid sequence with the av gene of other animals. The 3141bp cDNA of bovine integrin alphav encodes a polypeptide of 1046 amino acids consisting of a 30-residue putative signal peptide, a 955-residue ectodomain, a 29-residue transmembrane domain, and a 32-residue cytoplasmic domain. The ectodomain contains 11 potential N-linked glycosylation sites (NXT/NXS), 2 calcium binding domains (DXD/N] XDGXXD) and 18 cysteine residues. The nucleotide sequence similarities of integrin alphav between pig and cattle, human, rheses monkey, house mouse, chicken, dog are 93.3%, 91.5%, 91.4%, 85.6%, 73.2% and 89.9% respectively; and the amino acid sequence similarities are 96.3%, 94.6%, 94.1%, 90.8%, 81.6% and 93.8%, respectively. The alphav gene of cattle and pig exhibited the highest sequence homology. It is possible that host tropism of FMDV may related to divergence in receptors among different species.
%K 口蹄疫病毒
%K 病毒受体
%K 猪αv基因
%K 序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=591A98727731A2ABC0E702E964E44873&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=3395C64999261B75&eid=602F518C16859E7D&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=20