%0 Journal Article
%T MOLECULAR DESIGN OF IL-15 AND ITS MUTANTS<br>人白介素15及突变体的分子设计
%A SU Qin
%A FENG Jian-nan
%A WANG Jiaxi
%A <br>苏琴
%A 冯健男
%A 王嘉玺
%J 生物物理学报
%D 2000
%I 
%X The three dimensional (3-D) structure of IL-15 and its two mutants (4 amino acids-deleted at N-terminal or 3 amino acids -deleted at C-terminal) are constructed by means of computer-guided homology modeling techniques with the crystal structure of IL-2 as a template. The structures are optimized with molecular mechanism and molecular dynamics under CVFF-force field. Furthermore, the similarity and difference of the three proteins in biological activities are predicted based on the analysis of residues hydrophilic and hydrophobic characteristics and surface electrostatic potential distribution of these proteins. IL-15 and its two mutants are expressed in E.coli and their bioactivities are tested with CTLL-2 proliferation assay and LAK-induction assay. The results show that N-terminal mutant loses CTLL-2 proliferation stimulation activity but keeps the LAK-induction activity from PBMC,while C-terminal mutant increases both of the two bioactivities. These results correspond to the predictions.
%K IL-15
%K Homology modeling
%K Surface electrostatic potential distribution<br>人白介素15
%K 同源模建
%K 突变体
%K 空间结构
%K 质疫因子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=70C60361FCA690D9&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=85002451B65CE0D1&eid=AA5FB09E1F81059E&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=1&reference_num=1