%0 Journal Article
%T N01WA-735E, a Human Leukocyte Elastase Inhibitor from Metabolites of Microorganisms<br>微生物来源的HLE抑制剂N01WA-735E的研究
%A WEI Ya-Shan
%A ZHANG Hu
%A LU Xin-Hu
%A DONG Yue-Sheng
%A ZHAO Bao-Hua
%A <br>魏亚闪
%A 张华
%A 路新华
%A 董悦生
%A 赵宝华
%J 生物工程学报
%D 2007
%I 
%X Human leukocyte elastase is an important selection target of inflammation and cancer. In this paper, a high throughput screening model was established for screening human leukocyte elastase inhibitors from thousands of strains of actinomycetes. As a result, a strain, N01WA-735 with potent suppression activity was isolated. Firstly, the strain N01WA-735 was identified as Streptomyces according to morphology and biochemical analysis. The Streptomyces N01WA-735 was processed by solvent extraction, silica column chromatography, Sephadex LH-20 column chromatography and crystallization to get a pure active compound named N01WA-735E. Its chemical structure was elucidated as the same as that of the compound named BE-52440A by physicochemical properties and spectral data of UV, MS, 1H-NMR and 13C-NMR respectively. The compound showed a strong inhibitory activity against human leukocyte elastase with IC50 of 3.02 micromol/L. The compound is reported as a human leukocyte elastase inhibitor for the first time.
%K BE-52440A<br>微生物
%K 炎症
%K 人白细胞弹性蛋白酶抑制剂
%K 链霉菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=32625373D9A27DB565D85A4572C7AF22&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=1AA557EFF1C6B447&eid=CA10C709B736BBEA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15