%0 Journal Article
%T Cloning of Distinguishing DNA Sequences of Gastrodia elata Blume and Application of Them in Identifying Gastrodia Tuber<br>天麻特异DNA序列的克隆及其在天麻鉴定中的应用
%A TAO Jun
%A FU Tie-Xiang
%A LUO Zhi-Yong
%A WEN Li
%A WANG Zhi-Cheng
%A SHU Xiao-Shun
%A LIU Shui-Ping
%A TAO Yao
%A HU Wei-Xin
%A <br>陶钧
%A 傅铁祥
%A 罗志勇
%A 文李
%A 王志成
%A 舒孝顺
%A 刘水平
%A 陶垚
%A 胡维新
%J 生物工程学报
%D 2006
%I 
%X Gastrodia elata Bl. is a famous and costful traditional Chinese medicine. Their genomic DNA fingerprints were investigated using a modified Randomly Amplified Polymorphic DNA method. DNA fragments common to all or to fine populations were identified and recovered. Five DNA fragments were proven not to be reported through DNA cloning, PCR identifying, nucleotide sequencing and bioinformatics analyses and were received in and recorded by NCBI GenBank. Gastrodine contents of the Gastrodia tuber samples were determined using high performance liquid chromatography technique. The distribution of the five DNA fragments in 9 Gastrodia elata Blume populations and the correlation with gastrodine content were studied. The results show the distribution of these DNA sequences varied greatly among the populations whereby DNA Sequence 1 was the common and distinguishing molecular marker for all the populations studied and DNA Sequence 2 may relate to higher gastrodine content. In conclusion, these DNA marker sequences can be employed to identify genuine gastrodia tubers, better varieties and optimize their selection and cultivating.
%K DNA molecule marker
%K Gastrodia elata Bl
%K gastrodine
%K bioinformatics analyses<br>DNA分子标记
%K 天麻
%K 天麻素
%K 生物信息学分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7EB0FF5594540ACE&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=E158A972A605785F&sid=D5970ECA7D10A7B1&eid=E543FC2C7CA75C74&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=11