%0 Journal Article
%T Application of Glycosyl Fluorides in the Study on Glycosidases<br>氟代糖在糖苷酶研究中的应用
%A LU Li-Li
%A XIAO Min
%A ZHAO Han
%A WANG Peng
%A QIAN Xin-Min
%A <br>卢丽丽
%A 肖敏
%A 赵晗
%A 王鹏
%A 钱新民
%J 生物工程学报
%D 2006
%I 
%X Glycosyl fluorides are becoming increasingly important molecules for the study on glycosidases. Firstly, glycosyl fluorides act as substrates for glycosidases hydrolysis. Scecondly, the installation of fluorine elsewhere on the carbohydrate ring modifies the properties of the glycosyl fluoride so that the resultant compounds act as mechanism-based inhibitors to label enzymes in the active site, allowing identification of the catalytic nucleophile. Furthermore, glycosyl fluorides also act as donors for transglycosylation by retaining glycolides. Finally, glycosyl fluorides of the wrong anomeric configuration could be used by retaining glycosidase mutants such as glycosynthases and thioglycosynthases to synthesize carbohydrate with high yields(normally 60% to approximately 90%). Fundamental and applied research in biology, glycobiology and nanobiotechnology would benefit from the possibility of synthesizing tailor-made oligo-/poly-saccharides.
%K glycosyl fluorides
%K glycosidases
%K mechanism-based inhibitors
%K carbohydrate synthesis
%K glycosynthases
%K thioglycosynthases<br>氟代糖
%K 糖苷酶
%K 抑制剂
%K 糖类合成
%K 糖苷合成酶
%K 硫代糖苷合成酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=39BCE61B84E4A45C&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=38B194292C032A66&sid=381FB4265090A8E0&eid=5DCBAAB000A70168&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=49