%0 Journal Article
%T Acute Toxicity and Immunoprotection of Recombinant ApxI Toxin of Actinobacillus pleuropneumoniae in Mice<br>重组猪胸膜肺炎放线杆菌毒素ApxI对小鼠的急性毒性和免疫保护性研究
%A YAN Ke-Xia
%A LIU Jian-Jie
%A ZHOU Rui
%A WU Bin
%A LIU Wei-Hong
%A CHEN Huan-Chun
%A <br>严克霞
%A 刘建杰
%A 周锐
%A 吴斌
%A 刘维红
%A 陈焕春
%J 生物工程学报
%D 2006
%I 
%X Acute toxicity and immunoprotection of Actinobacillus pleuropneumoniae (APP) ApxI toxin recombinant proteins (include crude inclusion bodies and refolded recombinant protein) were evaluated in mice, and compared with the natural ApxI extracted from culture supernatant of APP serotype 10. In the acute toxicity experiment, the three proteins were intraperitoneally injected into Kunming mice in a dose of 200microg per mouse. The body and organ weight, heamatological and biochemical indexes were examined at 24h, 7 days and 14 days post administration. There was no death after the intraperitoneal administration of the three proteins, and no significant change was found in the body weight, organ indexes, heamatological and biochemical indexes. To study their immunoprotection, the three proteins were emulsified with Freund's adjuvant respectively and vaccinated the mice twice with a 2-week of interval. Two weeks after the second vaccination, the mice were challenged intraperitoneally with a lethal dose of APP serotype 10 (1.09 x 10(8) cfu), and serums were examined by an ApxI-specific ELISA. The results revealed that the recombinant protein had a good immunogenicity and could induce protection immune reaction.
%K Actinobacillus pleuropneumoniae
%K Recombinant ApxI
%K Mouse
%K Acute toxicity
%K Immune protection<br>胸膜肺炎放线杆菌
%K 重组apxIA
%K 小鼠
%K 急性毒性
%K 免疫保护性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F27BC81031F89C90&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=8BD23BD67BF01A5C&eid=09ABD5535D9B6D45&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=11