%0 Journal Article
%T Preparation and Performance of Chitosan-oligosaccharides/DNA complex Nanoparticles<br>纳米级壳寡糖/DNA复合物的制备和性能研究
%A ZHU Rong-Rong
%A HUANG Jin
%A WANG Shi-Lon
%A SUN Xiao-Yu
%A <br>钱君律
%A 朱融融
%A 黄进
%A 汪世龙
%A 孙晓宇
%J 生物工程学报
%D 2007
%I 
%X Comparing to Chitosan, Chitosan-oligosaccharides have several special functions, such as water-soluble, antitumor activity, immunostimulating effects, and antimicrobial activity. The chitosan-oligosaccharide, the molecular weight of which was about 5000, was used as research model. According to the agarose gel electrophoresis and UV spectrophotometer it was proved that electrostatic interaction was playing a very important role in the formation process of chitosan-oligosaccharide/DNA complex. The potential of adsorbing DNA on chitosan-oligosaccharide was analyzed by gel electrophoresis and UV spectrophotometer, and it was indicated that chitosan-oligosaccharide can improve the storage and structure stability of DNA. To check its protection ability to DNA by DNase I digestive experiment, the result showed that chitosan-oligosaccharide could load with plasmid effectively and protect DNA from being digested by DNase I. It was proved that chitosan-oligosacchide was safe and effective for gene delivery and will have a very good future in the field of gene therapy.
%K chitosan-oligosaccharide
%K pEGFPC-1 plasmid DNA
%K gene delivery
%K gel electrophoresis
%K UV spectrophotometer<br>纳米级壳寡糖
%K pEGFPC-1质粒DNA
%K 基因载体
%K 凝胶电泳法
%K 紫外光谱法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EEC5C47B804A5D8D&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=E158A972A605785F&sid=6D237E9625601349&eid=04EA291949415E08&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14