%0 Journal Article
%T Expression of Recombinant Human BMP6 in CHO Cells by Fused to the Signal Peptide and Propeptide of Another Homologue Protein<br>利用同源蛋白的信号肽和前肽序列在CHO细胞中表达重组人BMP6
%A YAN Ji-Dong
%A YANG Shuang
%A L Shu-Jun
%A LEI Rong-Yue
%A ZHU Tian-Hui
%A <br>闫继东
%A 杨爽
%A 吕树军
%A 雷荣悦
%A 朱天慧
%J 生物工程学报
%D 2007
%I 
%X BMP6 is a member of TGF-beta superfamily, represent more effective osteogenic activity. Two recombinant plasmids were constructed to expression rhBMP6 in mammalian cells, one contained the cDNA encoding the signal peptide, propeptide and mature peptide of human BMP6, wich was named pcDNA-BMP6, the other contained the recombinant DNA encoding the signal peptide, propeptide of human BMP2 and the mature peptide of BMP6, which was named pcDNA-BMP2/6. Transient expression in Cos7 cells demonstrated that the pcDNA-BMP2/6 produced more rhBMP6 than pcDNA-BMP6. For stable expression, the CHO-dhfr- cells were transfected with pcDNA-BMP2/6 and pSV2-dhfr, then screened by G418 and treated with MTX for targeting gene amplification. The partially purified rhBMP6 by heparin affinity chromatography was shown to possess bone induction activity tested by the induction of alkaline phosphatase activity in C2C12 cells.
%K BMP6<br>中华仓鼠卵巢细胞
%K 重组表达
%K 骨诱导作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C3A14E45F3768BAF&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=09AA1448D1EAF9C1&eid=84A93BA251D28205&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=12