%0 Journal Article
%T Study on Application of rep-PCR Fingerprint in Rapid Identification of Beer-spoilager<br>Rep-PCR应用于快速鉴定啤酒污染菌的研究
%A ZHU Lin-Jiang
%A ZHENG Fei-Yun
%A ZHAO Ya-Zhou
%A XING Xiang-Nan
%A LI Qi
%A GU Guo-Xian
%A <br>朱林江
%A 郑飞云
%A 赵亚洲
%A 邢香楠
%A 李崎
%A 顾国贤
%J 生物工程学报
%D 2006
%I 
%X The application potential of rep-PCR in typing beer-spoilage isolates was studied. The effects of different factors, including DNA templates and primers, on the quality and reproducibility of fingerprints were investigated. The CTAB protocol was shown to be the feasible method for DNA extraction. Primers BOXA1R and (GTG)5 were used in rep-PCR, and the PCR products were sequenced to identify strains isolated from two breweries. Rep-PCR fingerprint profiles were obtained by using GelCompar II software. Cluster analysis showed that the isolates belonging to Lactobacillus brevis, L. buchneri, L. casei/paracasei, L. plantarum are divided into 2 or 3 subgroups. In addition, the two rep-PCR fingerprint profiles complemented with each other in typing these isolates. Combining the similarity coefficient cut-off (SCC) of species, 9 unknown isolates were identified rapidly by using both fingerprint databases. The results indicate that rep-PCR is a simple, reliable and promising method for rapid identification of beer-spoilager.
%K rep-PCR
%K BOXA1R
%K (GTG)_5
%K similarity coefficient cut-off
%K beer-spoilager<br>rep-PCR
%K BOXA1R
%K (GTG)_5
%K 相似系数阈值
%K 啤酒腐败菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8B13DEABB16CD778&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=B31275AF3241DB2D&sid=4D5C5090B0F83CF9&eid=E947FD4445DA7BA0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=13