%0 Journal Article
%T Study on the Repeated Batch Fermentation of Lipase by Immobilized Rhizopus<br>固定化根霉发酵生产脂肪酶
%A YANG Xue-HaoTAN Tian-Wei
%A <br>杨学昊
%A 谭天伟
%J 生物工程学报
%D 2004
%I 
%X Repeated batch fermentation with Rhizopus arrhizus immobilized by polyurethane was optimized in details including the time to replace, the volume of the replaced medium and the optimal composition of the medium to replace. Immobilized cells showed stability for repeated use. Immobilized cells could be used for 9 batches, lasting 140h in flask and 6 batches in 5L fermenter when the substitute culture medium consisted of (%): soybean flour 3.0, earthnut oil 0.5 and the liquid was replaced by 40% . The time for fermentation was reduced largely and the lipase productivity was 3 - 6 times as high as that in batch fermentation.
%K lipase
%K immobilization
%K repeated-batch fermentation
%K Rhizopus arrhizus<br>脂肪酶，
%K 固定化，
%K 根霉，
%K 连续发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A8E642FFA3D984BE&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=0B39A22176CE99FB&sid=03E56C113B4E5A88&eid=11CEECA6DA9E4AC5&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=7&reference_num=28