%0 Journal Article
%T Study of Fusion Protein and Attachment Glycoprotein of Nipah Virus Expressed in Recombinant Baculovirus<br>重组杆状病毒表达尼帕病毒融合蛋白和受体结合蛋白的研究
%A Wang Xi-Jun
%A Hu Sen
%A Ge Jin-Ying
%A Wang Qing-Hua
%A Qin Li-Ting
%A Bu Zhi-Gao
%A <br>王喜军
%A 胡森
%A 葛金英
%A 王清华
%A 秦立廷
%A 步志高
%J 生物工程学报
%D 2006
%I 
%X In this study, Recombinant baculoviruses rBac-NF and rBac-NG were generated for expressing F and G proteins Nipah virus (NiV) . The expression of recommbinant G (rNG) and F (rNF) protein in rBac-NF and rBac-NG infected cells were confirmed by western-blot. Both rNG and rNF showed sensitive and specific antigenic reaction to rabbit serum anti-Nipah virus in indirect immunofluorescence detection and indirect ELISA. Immunization with rBac-NF and rBac-NG infected insect cells elicited G ad F protein specific antibody responses in mice. Furthermore, the G ad F specific antibodies could neutralize the infectivity of the VSVdeltaG* F/G, the NiV F and G envelope glycoproteins psudotyped recombinant Vesicular Stomatitis Virus expressing green fluorescence protein. The results demonstrated F and G protein expressed by the recombinant baculoviruses could be safe economic diagnostic antigens for the surveillance and monitoring of NiV and promising subunit vaccines for the prevention of NiV.
%K Nipah virus
%K fusion protein
%K attachment glycoprotein
%K recombinant baculovirus<br>尼帕病毒
%K 融合蛋白
%K 受体结合蛋白
%K 重组杆状病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF478385730D9839&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=38B194292C032A66&sid=6B3068A7C27BD349&eid=D1D63D047E37A053&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=20