%0 Journal Article
%T Immobilization of Lipase by Chemical Modified of Chitosan<br>改性壳聚糖固定化脂肪酶的研究
%A HU Wen-Jing
%A TAN Tian-Wei
%A WANG Fang
%A GAO Yang
%A <br>胡文静
%A 谭天伟
%A 王芳
%A 高阳
%J 生物工程学报
%D 2007
%I 
%X Lipase (EC3.1.1.3) from Candida sp.99-125 was immobilized on chitosan by chemical covalence. Lipase was first immobilized to chitosan beads by activating its hydroxyl groups with carbodiimide followed by cross-linking more lipase to the amino groups with glutaraldehyde. In this article, different factors that influenced the immobilization were investigated, and the optimum conditions were ascertained. Comparative studies of organic solvent and thermal stability between free lipase and immobilized lipase were conducted. Immobilization enhanced the lipase stability against changes of temperature and organic solvent. Immobilization lipase can be reused in the synthesis system of palmitate hexadecyl. Operational stability tests indicated that the immobilized lipase occurs after 16 consecutive batches, the conversion rate remained 85%. Such results revealed good potential for recycling under esterification system.
%K immobilize
%K lipase
%K chitosan
%K palmitate hexadecyl<br>固定化
%K 脂肪酶
%K 壳聚糖
%K 棕榈酸十六酯
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=70F9C5DDB2CE4E37&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=E158A972A605785F&sid=06DAE5E1DF7D0B6A&eid=93661EC4C0CCEA67&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11