%0 Journal Article
%T Studies on Antigencity of Human Immunodeficiency Virus Type 1(HIV-1) External Glycoprotein as well as Its Expression in Pichia pastoris<br>HIV-1膜蛋白在甲醇型酵母中的表达和抗原性的研究
%A ZHAO Li-Hui
%A YU Xiang-Hui
%A JIANG Chun-Lai
%A WU Yong-Ge
%A SHEN Jia-Cong
%A KONG Wei
%A <br>赵丽辉
%A 于湘晖
%A 姜春来
%A 吴永革
%A 沈家骢
%A 孔维
%J 生物工程学报
%D 2007
%I 
%X Based on the computer simulation, we analyzed hydrophobicity, potential epitope of recombined subtypes HIV-1 Env protein (851 amino acids) from Guangxi in China. Compared with conservative peptides of other subtypes in env protein, three sequences (469-511aa, 538-674aa, 700-734aa) were selected to recombine into a chimeric gene that codes three conservative epitope peptides with stronger antigencity, and was constructed in the yeast expression plasmid pPICZB.Chimeric proteins were expressed in Pichia pastoris under the induction of methanol, and were analyzed by SDS-PAGE and Westernblot. The results showed that fusion proteins of three-segment antigen were expressed in Pichia pastoris and that specific protein band at the site of 40kD was target protein, which is interacted with HIV-1 serum. The target proteins were purified by metal Ni-sepharose 4B, and were demonstrated to possess good antigenic specificity from the data of ELISA. This chimeric antigen may be used as research and developed into HIV diagnostic reagents.
%K Pichia pastoris
%K env gene
%K chimeric gene
%K antigenic specificity<br>毕赤酵母
%K env基因
%K 嵌合基因
%K 抗原特异性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=033F175251858BA4&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=D0E8F9CBDBE0070C&eid=FCACFF68346F8D4F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14