%0 Journal Article
%T Detection of Recombinant Lysostaphin Using Antibody Sandwish Enzyme-linked Immunoadsorbent Assay<br>抗体夹心酶联免疫吸附法测定重组溶葡萄球菌酶研究
%A HUANG Qing-Shan
%A ZHANG Ji-En
%A WU Hong-Yu
%A MO Yun-Jie
%A <br>黄青山
%A 张继恩
%A 吴宏宇
%A 莫云杰
%J 生物工程学报
%D 2007
%I 
%X The double-antibody-sandwich enzyme-linked immunoadsorbent assay (ELISA) for detection of rLysostaphin in humans had been developed and established through this study. rLysostaphin of high purity (>95%) produced in Shanghai Hi-Tech United Bio-Technological Research & Development Co., Ltd (SHUBRD) was used to produce a rabbit anti-rLysostaphin polyclonal antibody. The standard curve of rLysostaphin polyclonal antibody that was constructed showed that the lowest range of detection was found at 0.98 ng of rLysostaphin/mL, and the curve exhibited linearity preferably from 0.98 to 500 ng of rLysostaphin/mL. When three serum samples of the same batch were assayed for 6 replicates, and more 3 samples from different batches for 6 replicates, the average intra-assay and inter-assay coefficient variances (CV) were 6.4% and 6.5%, respectively. The relative recovery rate was 98.6% when quantitative standard antigens were added to the serum. The present method for detection of rLysostaphin in serum is specific, highly sensitive, highly precise, and exhibited a low CV and will be helpful in the further study of rLysostaphin pharmacokinetics and holds promise in clinical applications.
%K lysostaphin
%K polyclonal antibody
%K ELISA<br>溶葡萄球菌酶
%K 多克隆抗体
%K 酶联免疫吸附测定法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C5241981E9A891E5&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=7555FB9CC973F695&eid=CDEBD1ACE0A4C1C1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=7