%0 Journal Article
%T Study on Isoflavone Active Aglycone Preparation by Immobilized β-glucosidase from Aspergillus niger<br>固定化黑曲霉β-葡萄糖苷酶制备染料木素活性苷元的研究
%A PAN Li-Hu
%A LUO Jian-Ping
%A JIANG Shao-Tong
%A <br>潘利华
%A 罗建平
%A 姜绍通
%J 生物工程学报
%D 2007
%I 
%X With sodium alginate as a carrier and glutaraldehyde as the crosslinking agent, an improved immobilization method of beta-glucosidase for production of soybean genistein was developed. As compared with entrapment or entrapment-crosslinkage, crosslinkage-entrapment that beta-glucosidase was treated with glutaraldehyde and then entrapped in sodium alginate remained high loading efficiency and activity recovery, Effects of bead sizes, concentrations of alginate and glutaraldehyde as well, on the loading efficiency and activity recovery were assessed. When compared with the free enzyme, the optimum temperature, pH value and Km of the immobilized beta-glucosidase were respectively shifted from 50 degrees C to 40 degrees C, 4.5 to 4.0 and 2.57 microg/mL to 2.02 miocrog/mL. The stabilities of the immobilized beta-glucosidase were considerably better than that of the native enzyme. The immobilized beta-glucosidase was employed to genistein production, 84.94% of the activity and 56.04% of conversion were kept after consecutive use of 6 times.
%K β-葡萄糖苷酶
%K 海藻酸钠
%K 固定化酶
%K 黑曲霉
%K 大豆异黄酮
%K 活性苷元
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=85C200A8FEE31A230F4443D9CA1C5B9B&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=6B1D468AD7B8ADD8&eid=941A3E905B9F2AD9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=17