%0 Journal Article
%T Screening for Peptides of Anti-rotavirus by Phage-displayed Technique<br>噬菌体展示技术筛选抗轮状病毒多肽的试验研究
%A YAO Ning
%A YAO Lun-Guang
%A ZHANG Xiang-Man
%A GUO Tai-Lin
%A KAN Yun-Chao
%A <br>姚宁
%A 姚伦广
%A 张详满
%A 郭泰林
%A 阚云超
%J 生物工程学报
%D 2007
%I 
%X In this study, a 15-mer phage display peptide library was employed to pan against human rotavirus immobilized on solid phase. 4 different peptides were selected and could bind with rotavirus particles specifically. Plaque reduction neutralization test and MTT analysis results indicated that 3 of the peptides can inhibit rotavirus infecting in vitro. A peptide which sequence is QSNPIHIITNTRNHP showed the best efficiency--93% neutralization infectivity. Two other peptides, A and B, showed 40% and 50% neutralization infectivity respectively. Amino sequence analysis results indicate the 3 peptides containing 2 conserved motifs: SNPIHII and NIP. No putative trypsin hydrolysis site was found in C peptide, however, 4 and 3 potential sites were found in A and B peptides respectively. Using trypsin inhibitor, both A and B peptides showed the similar antiviral effect as that of C peptide. It suggests that the intactness of the 2 conserved motifs play an important role in counteracting virus infection. According to the results of this study, peptide C is hopeful to be exploited as an antiviral peptide drug.
%K rotavirus
%K phage display
%K antiviral peptide
%K plaque inhibition<br>轮状病毒
%K 噬菌体展示
%K 抗病毒多肤
%K 空斑抑制
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B3C5620E38816E7A&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=1E9426A299DC9FFD&eid=A53D7AA35F9929AF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11