%0 Journal Article
%T Effect of Phosphate on Growth and Polysaccharide Production by Suspension Cultures of Protocorm-like Bodies of Dendrobium huoshanense<br>磷对霍山石斛类原球茎悬浮培养细胞生长和多糖合成的影响
%A JIANG Shao-Tong
%A WEI Ming
%A LUO Jian-Ping
%A <br>姜绍通
%A 魏明
%A 罗建平
%J 生物工程学报
%D 2006
%I 
%X The effect of inorganic phosphate on cell growth, accumulation of polysaccharides together with nutrient utilization was investigated in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100g/L cells. The results indicate that the optimal concentration of phosphate in medium for cell growth of protocorm-like bodies of Dendrobium huoshanense was 2.5mmol/L and biomass was 496.5g/L (fresh weight). Phosphate was a limited factor for cell growth and there was relationship between the levels of intracellular phosphate and cell growth. 2.5mmol/L of medium phosphate was beneficial to the absorption of carbohydrate and nitrate source. 0.312mmol/L of medium phosphate was better for accumulation of polysaccharides and production of polysaccharides was 2.22 g/L at 36 d.
%K Dendrobium huoshanense
%K protocorm-like bodies
%K suspension culture
%K initial phosphate concentrations
%K polysaccharides<br>霍山石斛
%K 类原球茎
%K 悬浮培养
%K 起始磷浓度
%K 多糖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=01D7D6518EFFD6D1&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=E158A972A605785F&sid=9E7C0CB25117E09B&eid=54E527C5B72E59D8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=18