%0 Journal Article
%T Large Recombinant Protein Displayed on Filamentous Phage Surface and its Interaction with Small Molecule<br>大分子量重组蛋白在丝状噬菌体表面的展示表达及其与小分子相互作用的初步研究
%A KONG Bo
%A LIU Hui
%A YANG Sheng-Li
%A MA Wei-Jun
%A <br>孔波
%A 刘惠
%A 杨胜利
%A 马维骏
%J 生物工程学报
%D 2006
%I 
%X Recombinant proteins were expressed as fusions with the phagemid system of pHEN-KM13 and the characteristics and activities of the fusion proteins displayed on the surface of filamentous phage were studied. The altered titer of rescued phages from the phagemid system after trypsin treatment indicated the relative quantity of the phages displaying fusion proteins. The rescued phages displayed foreign proteins could keep the bacterial infection ability, while the bald phage without foreign protein displayed on its surface was sensitive to trypsin treatment and lost the bacterial infection ability. To determine the upper limit for filamentous phage display, four recombinant proteins, glutathione-S-transferase and glutathione-S-transferase fused with three various size peptide linkers, were fused to N terminus of capsid protein gp3 and rescued by helper phage KM13. The rescued phages which displayed fused protein with the size of 40kD or less maintain the infection ability. To assay the activity of the phage displayed protein, the known small molecule probe was used in the interaction study with protein incorporated on phage surface. Results showed that the glutathione-S-transferase on phage surface still bound to glutathione specifically. It indicated that the glutathione-S-transferase displayed on phage surface was correctly folded and functionally active. The results demonstrated that it was feasible to use small molecule probes to interact with the protein displayed on phage surface. In turn, the method described here also demonstrated that phage display system could be utilized to investigate the interactions between protein and small molecules.
%K phage
%K surface display
%K protein-small molecule interaction
%K protein expression
%K activity assay<br>噬菌体
%K 表面展示
%K 蛋白质与小分子相互作用
%K 蛋白质表达
%K 活性检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9ED0BBBB53B2FEC6&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=2A8D03AD8076A2E3&eid=C5154311167311FE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=13