%0 Journal Article
%T Over-expression in Escherichia coli and Purification of Nucleocaspid and Membrane Protein of SARS Coronavirus<br>SARS病毒核衣壳蛋白、膜蛋白在大肠杆菌中的高效表达和纯化
%A YI Yan-Ping
%A LI Chu-Fang
%A SHI Yu-Ling
%A LI Lin-Hai
%A LI Ping
%A HUANG Wei
%A WANG Sheng-Qi
%A MA Qing-Jun
%A CAO Cheng
%A <br>易艳萍
%A 李楚芳
%A 石玉岭
%A 李林海
%A 李平
%A 黄维
%A 王升启
%A 马清钧
%A 曹诚
%J 生物工程学报
%D 2003
%I 
%X Genes encoding nucleocaspid (N) and membrane(M)protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E.coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.
%K SARS coronavirus
%K nucleocaspid protein
%K membrane protein
%K expression<br>SARS病毒
%K 核衣壳蛋白
%K 膜蛋白
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5930D2CFAA7AAD4E&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=E158A972A605785F&sid=5A735990D5DE8BF4&eid=7D6CD8918B045FD4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=11&reference_num=8