%0 Journal Article
%T Soluble High-expression,Purification and Bioassay of IGFBP-3<br>IGFBP-3高效可溶表达、纯化及生物学活性初步研究
%A WU Chen
%A YAO Guang-Yin
%A ZOU Min-Ji
%A CHEN Guang-Yu
%A WANG Min
%A WANG Jia-Xi
%A XU Dong-Gang
%A <br>吴琛
%A 姚广印
%A 邹民吉
%A 陈光宇
%A 王旻
%A 王嘉玺
%A 徐东刚
%J 生物工程学报
%D 2007
%I 
%X cDNA for Insulin-like growth factor binding protein 3 was cloned and constructed a prokaryotic expression vector--pET-DsBA-IGFBP3. The construct was transformed into E. coli BL21 (DE3)plysS. The induced fusion protein (D-IGFBP3) was expressed successfully in soluble form. We obtained D-IGFBP3 the purify of which is over 95% after purification by His affinity chromatography. The product was identified by Western-blot. The cell assay showed that the obtained fusion protein can inhibit the growth of MCF-7 and bind with IGF-I in vitro.
%K IGFBP-3
%K soluble high-expression
%K purification
%K bioactivity<br>胰岛素样生长因子结合蛋白3(IGFBP-3)
%K 高效可溶表达
%K 纯化
%K 生物学活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FC94C63928FBC38C&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=4F0B2F798E08B761&eid=EE23223CE4332BD7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14