%0 Journal Article
%T Expression and Characterization of Fusion Protein tTF/SA as a Universal Effector for Targeting Blood Coagulation<br>凝血靶向通用效应因子tTF/SA融合蛋白的表达及活性鉴定
%A WANG Zhen
%A YAN Jiang-Hu
%A WANG Jie-Ping
%A XIE Lian-Ying
%A WU Na
%A <br>王臻
%A 颜江华
%A 王阶平
%A 谢莲英
%A 吴娜
%J 生物工程学报
%D 2007
%I 
%X To prepare a novel fusion protein (tTF/SA) as a universal effector for targeting therapy of blood coagulation and to analyze its biological activities. The fusion gene tTF/SA was constructed by PCR, then inserted into expression vector pET22 b ( ),and expressed inE. coli BL21 (DE_3). The fusion protein was purified using Nickel-affinity chromatography column. The activities of tTF moiety of the fusion protein were analyzed by clotting assay and FX activation assay, and the binding activities of Streptavidin(SA) to Biotin(B) were analyzed using ELISA. Results: The recombinant plasmid tTF/SA/pET22 b( ) with the correct sequence was obtained. The fusion gene tTF/SA was expressed with high yield in E. coli BL21(DE_3). The purified fusion protein retain the abilities of activating FX, inducing blood coagulation, and binding Biotin. The fusion gene tTF/SA was successfully expressed inE.coli BL21(DE_3). The recombinant tTF/SA proteins retain the activities of TF and SA. The multitarget therapy of selectively inducing thrombosis in tumor blood vessels can be achieved by the combination of tTF/SA, as a universal effector, and biotinlated carriers directing to tumor blood vessels.
%K truncated Tissue Factor
%K Streptavidin
%K fusion protein
%K thrombosis<br>截短组织因子
%K 链霉亲和素
%K 融合蛋白
%K 血管栓塞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C9453C3F5B6919FC&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=0B39A22176CE99FB&sid=AD16A18DBD734D13&eid=8B59EA573021D671&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=9