%0 Journal Article
%T Construction of Recombinant Fowlpox Virus Expressing Chicken IL-2 and Assay of Biologic Activity of the Product in vitro<br>表达鸡白细胞介素2重组鸡痘病毒的构建及其体外表达产物生物活性的检测
%A SHAO Wei-Xing PENG Da-Xin LU Jian-Hong WEI Dong-Ping
%A LIU Yu-Liang LIU Xiu-Fan
%A <br>邵卫星
%A 彭大新
%A 卢建红
%A 韦栋平
%A 刘玉良
%A 刘秀梵
%J 生物工程学报
%D 2004
%I 
%X In order to determine the adjuvant effects of the chicken IL-2 (ChIL-2) on new generation vaccines, ChIL-2 gene was amplified from ConA-stimulated chicken spleen cells by RT-PCR and was directionally inserted into fowlpox virus (FPV) transferring vector p1175 under the control of FPV early/late promoter (PE/L), resulting in recombinant transferring vector p1175IL2. Then the p1175IL2 plasmid was transfected into chicken embryo fibroblasts (CEF) pre-infected with wild type FPV to generate recombinant fowlpox virus expressing ChIL-2 (rFPV-IL2). By selection of blue plaques on the CEF, overlaid with agar containing X-gal, rFPV-IL2 was obtained and purified. The supernatant from CEF monolayer infected with rFPV-IL2 (M.O.I2.0) after 72 hours was detected for the production of ChIL-2 by XTT/PMS colorimetric assay. About 3.6 x 10(5) u/mL of specific ChIL-2 activity was determined. The results show that rFPV-IL2 can express ChIL-2 effectively. rFPV-IL2 provides us with an effective tool for studying avian immunology as well as a potential vaccine-enhancing agent.
%K interleukin-2
%K fowlpox virus
%K XTT/PMS<br>ConA，
%K 白细胞介素2，
%K 鸡痘病毒，
%K XTT/PMS
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C955A9FD2D47ADA4&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=58F693790F887B3B&eid=12DC19455C3A2FA8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=5&reference_num=15