%0 Journal Article
%T Construction and Expression of the Soybean Isoflavonoid Biosynthetic Pathway in Escherichia coli<br>大豆异黄酮代谢途径在大肠杆菌中的构建及表达
%A HAO Ji
%A MA Hui-Qin
%A DAI Ru
%A CHEN Shang-Wu
%A <br>郝佳
%A 马会勤
%A 代茹
%A 陈尚武
%J 生物工程学报
%D 2007
%I 
%X The natural isoflavones biosynthetic pathway is only limited in legumes plant. To study the isoflavone in bacteria by metabolic engineering requires transformation of multi-gene of the whole pathway into the host strain to resembling the expression and metabolism of the genes. The multi-gene transformation and expression strategy become necessary because of this. This article talks about the multi-gene transformation strategy using one or many vectors, taking the five genes of isoflavonoid biosynthetic pathway to E. coli. The recombinant bacteria carry five genes with two vectors, the whole Isoflavonoid biosynthetic pathway was constructed into E. coli. Fermented with L-tyrosine as substrate and IPTG as an inducer, the recombinant bacteria can produce a new isoflavone related metabolite showed on HPLC analysis profile.
%K isoflavonoid biosynthetic pathway
%K multi-gene transformation strategy
%K recombinant<br>异黄酮代谢途径
%K 多基因导引
%K 重组菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CD446982BCFEDCE3973EF4B20594B42C&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=92CE31955C2FC520&eid=5CC11A326E54A79A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=21