%0 Journal Article
%T Synthesis of Diacylglycerol Using Immoblized 1, 3-Regiospecific Lipase in Continuously Operated Fixed Bed Reactors<br>填充床反应器中酶法连续合成甘油二酯的研究
%A MENG Xiang-He
%A SUN Pei-Long
%A YANG Kai
%A He Rong-Jun
%A MAO Zhong-Gui Colloge of Biological & Environmental Engineering
%A Zhejiang University of Technology
%A Hangzhou
%A China School of Biotechnology
%A Southern Yangtze University
%A Wuxi
%A China
%A <br>孟祥河
%A 孙培龙
%A 杨开
%A 何荣军
%A 毛忠贵
%J 生物工程学报
%D 2005
%I 
%X Diacylglycerol, DAG, because of its multifunctional and nutritional properties, attracted considerable attention recently. Enzymatic synthesis of diacylglycerols from linoleic acid was investigated in a solvent-free reaction in a continuously operated fixed bed reactors containing Lipozyme RM IM. By appropriate manipulation of the fluid-residence time, the relative proportions of the various acylglycerols in the effluent stream can be controlled. In addition, the presence of excess glycerol is effective for the removal of water produced during the esterification reactions. Under the conditions of molar ratio of linoleic acid to glycerol of 0.5, the immoblized enzyme maintained high stability and allowed the reaction to continue for 10 days without significant deterioration in enzyme activity. It was determined that the conversion of fatty acid, content of 1,3-DAG and volume efficiency of reactor reached optima under the conditions: a packaged-bed reactor(with a ratio of packed length to inner diameter of 7.8), reacting temperature at 65 degrees C, molar ratio of linoleic acid to glycerol of 0.5, and feeding flow rate of 1.2 mL/min.
%K 1
%K d-d-Diacylglycerol
%K synthesis
%K lipozyme RM IM
%K fixed bed reactor
%K water removal<br>1
%K 3-甘油二酯
%K 合成
%K Lipozyme
%K RM
%K IM
%K 填充柱反应器
%K 脱水
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=3192BFBA07BE4A52&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=38B194292C032A66&sid=F10601728A1E9BEA&eid=9BA67A0B76A3DBA8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=19