%0 Journal Article
%T Effects of SCF, LIF and bFGF on Mouse Spermatogonial Stem Cells Proliferation in vitro<br>体外培养条件下SCF、LIF与bFGF对昆明白小鼠精原干细胞增殖的影响
%A YIN Ming
%A LI De-Xue
%A <br>尹明
%A 李德雪
%J 生物工程学报
%D 2002
%I 
%X The present study identified the favorable environment conditions for Spermatogomial stem cells in vitro according to their unique biological properties. Three growth factors, stem cell factor (SCF), leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF) were all found to independently contribute to the proliferation of mouse spermatogonial stem cell. The percentage of cell proliferation significantly enhanced by SCF at 30 ng/mL but decreased with heightening its combination after cultured 120 hours. The mice spermatogonial stem cells were significantly proliferated after 120 hours' culture with 10 ng/mL and 20 ng/mL (P<0.01) of LIF, between 20 ng/mL and 50 ng/mL (P<0.01) for bFGF. SCF and bFGF were significantly enhanced mice spermatogonial stem cells proliferation after these three factors combination. For LIF, no obvious effect was abserved.
%K spermatogonial stem cell
%K proliferation
%K growth factor
%K culture in vitro<br>小鼠精原干细胞，
%K 增殖，
%K 生长因子，
%K 体外培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CEDB2EF9D762BBF8&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=9409F3EB075DCD5B&eid=A8E9231F98774741&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=13&reference_num=10