%0 Journal Article
%T Virulence Genes of Agrobacterium tumefaciens and Analysis Function of its Biology<br>根癌农杆菌致病基因及其生物学功能分析
%A LU Xiao-Ping
%A XU Ya-Xiang Mineo Kojima
%A <br>陆小平
%A 许雅香
%A 小岛峰雄
%J 生物工程学报
%D 2003
%I 
%X Chromosomal virulence genes acvB, abvA, chvA of %Agrobacterium tumefaciens% were cloned with the technique of transposon 5 insertion. The chromosome genes are necessary for %Agrobacterium tumfaciens% absorbing to cell ular surface of plant, the adherence reaction can't be executed and result in losing the toxicity if mutations are occurred in some chromosome genes. The chromosome toxicity gene is inactivated due to transposon Tn5 be inserted and the accept ant cell infected with Agrobacterium tumefaciens can't cause tumor ultimately.This article briefly introduces the research way of thinking and strategy of this technique and the important roles of every gene, which are taken of in the process of T-DNA's form, transfer, integration, and expression %etc%. This article also gives a presumption to T-DNA's transport: The plant cell wall's porin may be T-DNA's natural channel.
%K transposon5
%K A
%K tumefaciens
%K virulence genes
%K T-DNA transport<br>转座子(Tn5)，
%K 农杆菌，
%K 致病基因，
%K T-DNA转移
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D63BF30BA0402CF7&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=B31275AF3241DB2D&sid=7004BE6E41AAF52C&eid=BBA8B1249CDAA6CE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=16