%0 Journal Article
%T Comparison of Adsorbent with Varying Arm Length and Ligand Density for the Purification of Recombinant Hepatitis B Virus Surface Antigen<br>间臂和丁基密度对疏水层析分离纯化重组乙肝病毒表面抗原的影响
%A LI Rui-Hong
%A LI Yan
%A BI Jing-Xiu
%A ZHAO Lan
%A ZHOU Wei-Bin
%A HUANG Yong-Dong
%A ZHANG Yan
%A SUN Li-Jing
%A WANG Hua-Jun
%A SU Zhi-Guo
%A <br>李瑞红
%A 李岩
%A 闭静秀
%A 赵岚
%A 周卫斌
%A 黄永东
%A 张焱
%A 孙李靖
%A 王化军
%A 苏志国
%J 生物工程学报
%D 2007
%I 
%X Novel hydrophobic absorbents were synthesized by immobilizing butyl derivative onto the highly cross-linked agarose beads manufatured in China, which are used as matrix. The effect of the spacer arm length (3C, 8C and 10C) and ligand density (from 13 to 45 micromol/mL) on the hydrophobicity were investigated using purified Hepatitis B surface antigen (HBsAg) expressed by CHO cell lines. Also considering the effects of salt concentration and pH on HBsAg recovery and purification factor, orthogonal experiment design method was used to evaluated the absorbents. The results showed the butyl-S absorbent with the spacer arm length of C8, the ligand density of 22 micromol/mL gel showed the best performance for the separation of HBsAg. Approximately 100% HBsAg recovery and 60 as purification fold were achieved by this media under the operating condition of pH 7.0 and 9% of salt concentrateion.
%K hydrophobic absorbent
%K spacer arm
%K ligand density
%K recombinant hepatitis B surface antigen
%K purification
%K orthogonal experiment design<br>疏水层析介质
%K 间臂
%K 配基密度
%K 重组乙肝病毒表面抗原
%K 分离纯化
%K 正交试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=78B5A9D7651B6582&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=E158A972A605785F&sid=15F713CDE16A589C&eid=C230D48775108CE3&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15